A lysR-type Regulator Is Involved in the Negative Regulation of Genes Encoding Selenium-Free Hydrogenases in the Archaeon Methanococcus Voltae

Mol Microbiol. 2004 Apr;52(2):563-71. doi: 10.1111/j.1365-2958.2004.03998.x.

Abstract

The archaeon Methanococcus voltae encodes two pairs of NiFe-hydrogenase isoenzymes. One hydrogenase of each pair contains selenium in the active site, whereas the other one is selenium-free. The gene groups for the selenium-free hydrogenases, called vhc and frc, are linked by a common intergenic region. They are only transcribed under selenium limitation. A protein binding to a negative regulatory element involved in the regulation of the two operons was purified by DNA-affinity chromatography. Through the identification of the corresponding gene the protein was found to be a LysR-type regulator. It was named HrsM (hydrogenase gene regulator, selenium dependent in M. voltae). hrsM knockout mutants constitutively transcribed the vhc and frc operons in the presence of selenium. A putative HrsM binding site was also detected in the intergenic region in front of the hrsM gene. Northern blot analysis indicated that the hrsM gene might be autoregulated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Archaeal Proteins / biosynthesis
  • Archaeal Proteins / chemistry
  • Archaeal Proteins / genetics
  • Archaeal Proteins / metabolism*
  • Base Sequence
  • Binding Sites
  • Chromatography, Affinity
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Regulation, Archaeal*
  • Hydrogenase / biosynthesis
  • Hydrogenase / chemistry
  • Hydrogenase / genetics*
  • Methanococcus / enzymology
  • Methanococcus / genetics*
  • Molecular Sequence Data
  • Operon
  • Plasmids
  • Promoter Regions, Genetic
  • Selenium / analysis
  • Selenium / metabolism
  • Sequence Analysis, DNA
  • Transcription, Genetic*
  • Transfection

Substances

  • Archaeal Proteins
  • DNA-Binding Proteins
  • nickel-iron hydrogenase
  • Hydrogenase
  • Selenium