Finb, a multiple zinc finger protein, represses transcription of the human angiotensinogen gene

Int J Mol Med. 2004 May;13(5):637-42.

Abstract

We previously identified a regulatory element at the 3'-downstream region of the human angiotensinogen (hANG) gene. Using this element as a probe by the Southwestern screening, we isolated a cDNA clone, encoding Finb, a transcriptional activator with multiple zinc finger domains. The N-terminal zinc finger domain of Finb bound to the GGATGG sequence within the regulatory element. Unexpectedly, Finb repressed transcription dependent on the regulatory element. Inspection of the 5'-flanking region in the hANG promoter identified the GGATGG-like elements, which prompted us to examine the effect of Finb on the hANG promoter activity. We also found the two Finb binding elements in the 5'-flanking region of the hANG gene by the gel shift assay, both of which were necessary for transcriptional repression of the hANG promoter. These findings suggest that Finb functions as a sequence-specific transcriptional repressor of the hANG gene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensinogen / genetics*
  • Base Sequence
  • Cell Line, Tumor
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / chemistry*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Down-Regulation / genetics*
  • Humans
  • Molecular Sequence Data
  • Promoter Regions, Genetic / genetics
  • Protein Binding
  • Repressor Proteins / chemistry
  • Repressor Proteins / metabolism*
  • Response Elements / genetics
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic / genetics*
  • Zinc Fingers*

Substances

  • DNA, Complementary
  • DNA-Binding Proteins
  • RREB1 protein, human
  • Repressor Proteins
  • Transcription Factors
  • Angiotensinogen