Centrosome maturation and duplication in C. elegans require the coiled-coil protein SPD-2

Dev Cell. 2004 Apr;6(4):511-23. doi: 10.1016/s1534-5807(04)00066-8.

Abstract

Centrosomes are major determinants of mitotic spindle structure, but the mechanisms regulating their behavior remain poorly understood. The spd-2 gene of C. elegans is required for centrosome assembly or "maturation." Here we show that spd-2 encodes a coiled-coil protein that localizes within pericentriolar material (PCM) and in the immediate vicinity of centrioles. During maturation, SPD-2 gradually accumulates at the centrosome in a manner that is partially dependent on Aurora-A kinase and cytoplasmic dynein. Interestingly, SPD-2 interacts genetically with dynein heavy chain and SPD-5, another coiled-coil protein required for centrosome maturation. SPD-2 and SPD-5 are codependent for localization to the PCM, but SPD-2 localizes to centrioles independently of SPD-5. Surprisingly, we also find that SPD-2 is required for centrosome duplication and genetically interacts with ZYG-1, a kinase required for duplication. Thus, we have identified SPD-2 as a factor critical for the two basic functions of the centrosome-microtubule organization and duplication.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence / genetics
  • Animals
  • Aurora Kinases
  • Base Sequence / genetics
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / metabolism*
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / isolation & purification*
  • Caenorhabditis elegans Proteins / metabolism*
  • Cell Cycle Proteins
  • Cell Differentiation / genetics
  • Centrioles / genetics
  • Centrioles / metabolism
  • Centrosome / metabolism*
  • DNA, Complementary / analysis
  • DNA, Complementary / genetics
  • Dyneins / genetics
  • Dyneins / metabolism
  • Microtubule-Organizing Center / metabolism*
  • Microtubules / genetics
  • Microtubules / metabolism
  • Mitosis / genetics*
  • Molecular Sequence Data
  • Protein Kinases / genetics
  • Protein Kinases / metabolism
  • Protein Structure, Tertiary / genetics
  • Protein-Serine-Threonine Kinases
  • Sequence Homology, Amino Acid
  • Spindle Apparatus / genetics
  • Spindle Apparatus / metabolism*
  • Xenopus Proteins

Substances

  • Caenorhabditis elegans Proteins
  • Cell Cycle Proteins
  • DNA, Complementary
  • SPD-2 protein, C elegans
  • Xenopus Proteins
  • Protein Kinases
  • zyg-1 protein, C elegans
  • AURKA protein, Xenopus
  • Aurora Kinases
  • Protein-Serine-Threonine Kinases
  • Dyneins

Associated data

  • GENBANK/AY340594