Four studies examined whether situational and individual differences in individuals' regulatory focus influence how intergroup bias is expressed emotionally and behaviorally. Consistent with past findings on promotion focus, these studies found evidence that participants' promotion focus, whether measured or manipulated, was related to how extensively they demonstrated bias toward their ingroup in terms of cheerfulness- and dejection-related emotions and approach-related behaviors. Consistent with past findings on prevention focus, these studies also revealed that participants' prevention focus was related to how extensively they showed bias against an outgroup in terms of quiescence- and agitation-related emotions and avoidance-related behaviors. The implications for the self-regulatory functions of intergroup bias are discussed.