Rapid detection protocol for filoviruses

J Clin Virol. 2004 May;30(1):94-9. doi: 10.1016/j.jcv.2003.09.004.


Background: The incidence of filovirus disease outbreaks has been increasing in recent years. Although there have been advances in the developments of diagnostics, field tests are rare. Apart from family members of infected patients, health care workers are at high risk of being infected during the initial phase of an outbreak. RT-PCR has been shown to be helpful in containing outbreaks.

Objectives: To develop Taqman-RT-PCR for the detection of Ebola-Zaire virus (EBOV-Z), Ebola-Sudan virus (EBOV-S) and Marburg virus (MBGV).

Study design: Quantitative Taqman-RT-PCRs for the detection of these viruses were developed and established on a portable Smartcycler TD.

Results and conclusions: All three assays were highly sensitive and specific. The mobility of the assay system may help to contain future outbreaks.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Ebolavirus / genetics
  • Ebolavirus / isolation & purification*
  • Hemorrhagic Fever, Ebola / diagnosis
  • Hemorrhagic Fever, Ebola / virology
  • Humans
  • Marburg Virus Disease / diagnosis
  • Marburg Virus Disease / virology
  • Marburgvirus / genetics
  • Marburgvirus / isolation & purification*
  • Molecular Sequence Data
  • Nucleocapsid Proteins / genetics
  • RNA-Binding Proteins / genetics
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Ribonucleoproteins / genetics
  • Sensitivity and Specificity
  • Viral Proteins / genetics


  • Nucleocapsid Proteins
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • Viral Proteins
  • nucleocapsid protein, Ebola virus
  • nucleoprotein, Marburg virus