Expression and localization of ecto-nucleotide pyrophosphatase/phosphodiesterase I-1 (E-NPP1/PC-1) and -3 (E-NPP3/CD203c/PD-Ibeta/B10/gp130(RB13-6)) in inflammatory and neoplastic bile duct diseases

Cancer Lett. 2004 Apr 30;207(2):139-47. doi: 10.1016/j.canlet.2003.11.002.


Ecto-nucleotide pyrophosphatase/phosphodiesterase-I enzyme (E-NPP) consists of three closely related molecules: E-NPP1, E-NPP2 and E-NPP3. We investigated the expression and localization of E-NPP1 and -3 in human inflammatory and neoplastic bile duct diseases. Immunohistochemically E-NPP1 was located on the apical cytoplasmic side of cancer cells, whereas E-NPP3 was located in the apical plasma membrane. Western blot analysis revealed that the expression of E-NPP3, but not E-NPP1, was higher in tumor tissues than in surrounding tissues and the specific form of the E-NPP3 protein was readily detected in the sera of bile duct carcinoma (BDC) patients. Furthermore, it was confirmed that E-NPP3 was associated with migration ability by using of NIH3T3 cells that stably transfected with E-NPP3 cDNA. These results suggest that E-NPP3 is involved in the infiltration of neoplastic BDC and is possible to be a tumor marker.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Bile Duct Neoplasms / enzymology*
  • Bile Duct Neoplasms / genetics
  • Bile Duct Neoplasms / pathology
  • Blotting, Western
  • Case-Control Studies
  • Cell Membrane / enzymology
  • Cell Movement
  • Cytoplasm / metabolism
  • DNA, Complementary / genetics
  • DNA, Complementary / metabolism
  • Humans
  • Immunoenzyme Techniques
  • In Situ Hybridization
  • Liver Cirrhosis, Biliary / enzymology*
  • Liver Cirrhosis, Biliary / genetics
  • Liver Cirrhosis, Biliary / pathology
  • Mice
  • NIH 3T3 Cells
  • Phosphoric Diester Hydrolases / genetics
  • Phosphoric Diester Hydrolases / metabolism*
  • Pyrophosphatases / genetics
  • Pyrophosphatases / metabolism*
  • RNA Probes
  • Transfection


  • DNA, Complementary
  • ENPP3 protein, human
  • RNA Probes
  • Phosphoric Diester Hydrolases
  • ectonucleotide pyrophosphatase phosphodiesterase 1
  • Pyrophosphatases