Adoptive transfer of anti-idiotypic T cells cure mice of disseminated B cell lymphoma

J Immunother. May-Jun 2004;27(3):227-31. doi: 10.1097/00002371-200405000-00007.

Abstract

There is extensive interest in idiotypic vaccination as a treatment of lymphoma. An alternative approach is the adoptive transfer of in vitro generated T cells. This strategy has been used to treat posttransplantation EBV-related diseases. The ability to generate in vitro T cells to peptides derived from immunoglobulin idiotypes raises the possibility of directly using such cells as a treatment of lymphoma. Investigating the adoptive transfer of specific T cells to idiotype derived peptides in a murine lymphoma model is therefore an important part of the clinical translation of this alternative approach. We have generated an idiotype-specific T cell line, able to recognise a defined, naturally processed idiotype-derived epitope. This line has been used to successfully treat mice with disseminated lymphoma supporting the clinical use of idiotype specific T cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics
  • Adoptive Transfer / methods*
  • Animals
  • Cell Division
  • Cell Line, Tumor
  • Chromium / chemistry
  • Enzyme-Linked Immunosorbent Assay
  • Epitopes / chemistry
  • Female
  • Immunoglobulin Fragments
  • Immunoglobulin Idiotypes / chemistry*
  • Immunoglobulins / chemistry
  • Immunotherapy / methods*
  • Immunotherapy, Adoptive
  • Interferon-gamma / chemistry
  • Lymphoma / immunology
  • Lymphoma, B-Cell / chemistry
  • Lymphoma, B-Cell / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Peptides / chemistry
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism
  • Time Factors

Substances

  • Epitopes
  • Immunoglobulin Fragments
  • Immunoglobulin Idiotypes
  • Immunoglobulins
  • Peptides
  • Chromium
  • Interferon-gamma