Escherichia coli producing CTX-M-2 beta-lactamase in cattle, Japan

Emerg Infect Dis. 2004 Jan;10(1):69-75. doi: 10.3201/eid1001.030219.

Abstract

From November 2000 to June 2001, Escherichia coli strains producing CTX-M-2 beta-lactamase were isolated from 6 (1.5%) of 396 cattle fecal samples and 2 (0.7%) of 270 surface swabs of cattle carcasses in Japan. The blaCTX-M-2 gene responsible for CTX-M-2 production was encoded on transferable plasmids, and the gene was transferred to E. coli CSH2 with a very high frequency (2 x 10(-4) to 6 x 10(-1) per donor cells) by conjugation. Random amplified polymorphic DNA analysis of nine isolates showed at least five different patterns. These findings suggest that CTX-M-2 producers might have originated from cattle through the use of cephalosporins such as ceftiofur and that cattle could be a reservoir of CTX-M-2-producing E. coli. Continuous and strategic surveillance of antimicrobial-resistant bacteria in livestock is essential to suppress further dissemination of these bacteria into society at large.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cephalosporins / pharmacology*
  • Escherichia coli / drug effects
  • Escherichia coli / enzymology*
  • Feces / microbiology
  • Japan
  • Microbial Sensitivity Tests
  • Random Amplified Polymorphic DNA Technique
  • beta-Lactamases / biosynthesis
  • beta-Lactamases / genetics*
  • beta-Lactamases / isolation & purification

Substances

  • Cephalosporins
  • beta-lactamase CTX-2
  • beta-Lactamases