[Rotavirus A infections and reinfections: genotyping and vaccine implications]

J Pediatr (Rio J). Mar-Apr 2004;80(2):119-22.
[Article in Portuguese]

Abstract

Objective: To identify rotavirus A and the most prevalent G and P genotypes in children with acute diarrhea, and to the describe the occurrence of rotavirus infection and reinfection.

Methods: Group A rotavirus specimens were obtained from fecal samples from children with acute diarrhea in Goiânia, state of Goiás, Brazil from July 2000 to October 2002. Rotavirus A positive children and a control group (children of the same age and sex, without diarrhea and with no evidence of rotavirus in the first fecal samples) were followed prospectively during one year. All rotavirus A positive samples were genotyped using RT-PCR/nested-PCR.

Results: A total of 77 group A rotavirus strains (37.2%) were identified in the diarrheic samples of 207 children. The following G genotypes were identified: G1 (62.3%), G9 (34.4%) and G4 (3.3%). With regard to P genotyping, 59% were characterized as P[8], 7.7% as P[6], 23.1% as P[6]+P[8], 7.7% as P[4]+P[8] and 2.6% as P[4]+P[8]. The following associations were observed: G1P[8] (77.8%), G9P[8] (11.1%), G4P[8] (5.6%) and G1P[6] (5.6%). No reinfection was observed in the 40 rotavirus A (+) children. However, but two of 40 children who were initially negative for this agent developed rotavirus infection during the same period.

Conclusions: The predominant G and P genotypes observed were similar to those found in new vaccines. No reinfection occurred during one-year of follow-up for any of the genotypes identified.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Case-Control Studies
  • Diarrhea / virology*
  • Female
  • Follow-Up Studies
  • Genotype
  • Humans
  • Infant
  • Male
  • Prospective Studies
  • Recurrence
  • Reverse Transcriptase Polymerase Chain Reaction
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification
  • Rotavirus Infections / complications
  • Rotavirus Infections / prevention & control*
  • Rotavirus Vaccines / therapeutic use

Substances

  • Rotavirus Vaccines