Objective: To identify rotavirus A and the most prevalent G and P genotypes in children with acute diarrhea, and to the describe the occurrence of rotavirus infection and reinfection.
Methods: Group A rotavirus specimens were obtained from fecal samples from children with acute diarrhea in Goiânia, state of Goiás, Brazil from July 2000 to October 2002. Rotavirus A positive children and a control group (children of the same age and sex, without diarrhea and with no evidence of rotavirus in the first fecal samples) were followed prospectively during one year. All rotavirus A positive samples were genotyped using RT-PCR/nested-PCR.
Results: A total of 77 group A rotavirus strains (37.2%) were identified in the diarrheic samples of 207 children. The following G genotypes were identified: G1 (62.3%), G9 (34.4%) and G4 (3.3%). With regard to P genotyping, 59% were characterized as P, 7.7% as P, 23.1% as P+P, 7.7% as P+P and 2.6% as P+P. The following associations were observed: G1P (77.8%), G9P (11.1%), G4P (5.6%) and G1P (5.6%). No reinfection was observed in the 40 rotavirus A (+) children. However, but two of 40 children who were initially negative for this agent developed rotavirus infection during the same period.
Conclusions: The predominant G and P genotypes observed were similar to those found in new vaccines. No reinfection occurred during one-year of follow-up for any of the genotypes identified.