Identification of an early-growth-response gene encoding a novel putative protein kinase

Mol Cell Biol. 1992 Sep;12(9):4164-9. doi: 10.1128/mcb.12.9.4164.

Abstract

Early-growth-response genes, also known as immediate-early genes, play important roles in regulating cell proliferation. We have identified a new type of early-growth-response gene product, a 77,811-Da putative serine/threonine kinase, which is highly inducible by serum and phorbol ester. mRNA encoding this putative kinase is markedly elevated within 1 h after treatment with mitogen, and this induction is synergistically increased by cycloheximide. Dexamethasone blocks serum induction of the kinase mRNA, as does transformation by v-Ki-ras. The kinase mRNA was detected in mouse brain, lung, and heart. This new putative kinase, which we term Snk, for serum-inducible kinase, showed similarity in its proposed catalytic domain to many other protein kinases; however, no other kinase showed enough sequence similarity with Snk to suggest the existence of a common function. Hence, Snk represents a new type of protein kinase involved in the early mitogenic response whose activity is transcriptionally and posttranscriptionally regulated.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • DNA
  • Gene Expression Regulation
  • Glucocorticoids / physiology
  • Male
  • Mice
  • Mitogens / pharmacology
  • Molecular Sequence Data
  • Organ Specificity / genetics
  • Protein Kinases / genetics*
  • Protein Kinases / metabolism
  • RNA, Messenger / metabolism
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic

Substances

  • Glucocorticoids
  • Mitogens
  • RNA, Messenger
  • DNA
  • Protein Kinases