Near-infrared FT-Raman spectra of the rat brain tissues

Neurosci Lett. 1992 Jul 6;141(1):47-52. doi: 10.1016/0304-3940(92)90331-z.

Abstract

Near-infrared Fourier transform (FT) Raman spectroscopy was applied to brain tissues in situ. The spectra were obtained from the cerebral cortex, white matter of the cerebrum, caudate-putamen, thalamus, synaptosomal fraction, and myelin fraction. High-quality Raman spectra in the 400 to 2940 cm-1 range were measured without interference of autofluorescence. Common spectral bands were assigned. The ratios of the intensity at 1664 (amide I), 1442 (CH2 deformation), 2885 (CH2 asymmetric stretching), 2938 cm-1 (CH3 symmetric stretching) could be used for differentiation between the gray and white matters.

MeSH terms

  • Animals
  • Brain Chemistry*
  • Fatty Acids / analysis
  • Fourier Analysis
  • Male
  • Myelin Sheath / chemistry
  • Nerve Tissue Proteins / metabolism
  • Rats
  • Rats, Inbred Strains
  • Spectrum Analysis, Raman
  • Synaptosomes / chemistry

Substances

  • Fatty Acids
  • Nerve Tissue Proteins