Recoverin as a paraneoplastic antigen in lung cancer: the occurrence of anti-recoverin autoantibodies in sera and recoverin in tumors

Lung Cancer. 2004 May;44(2):193-8. doi: 10.1016/j.lungcan.2003.10.006.


Using immunoblotting with recombinant recoverin as an antigen, we have examined 279 serum samples from individuals with small cell lung carcinoma (SCLC, 99 patients), non-small cell lung carcinoma (NSCLC, 44 patients), and non-malignant pulmonary disorders (86 patients) as well as sera from 50 healthy donors. Autoantibodies against recoverin (anti-Rc) were detected in sera from 15 patients with SCLC (15% of cases) and from 9 patients with NSCLC (about 20% of cases). Only two anti-Rc positive cases were detected in patients with non-malignant pulmonary disorders, while no such cases were found in healthy individuals. Immunohistochemical investigation of paraffin sections of 44 SCLC and 40 NSCLC tumors revealed recoverin-positive reaction in 30 SCLC (68%) and 34 NSCLC (85%) sections. Despite the high specificity (98%), the low sensitivity (less than 20%) does not allow serum anti-Rc to be considered as a valuable marker of lung cancer. However, taking into account the high occurrence of aberrant expression of recoverin in lung tumors, this PNA could be considered as a potential target for immunotherapy of lung cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Antigens, Neoplasm / analysis*
  • Autoantibodies / analysis*
  • Biomarkers, Tumor / analysis*
  • Calcium-Binding Proteins / analysis*
  • Carcinoma, Non-Small-Cell Lung / immunology*
  • Carcinoma, Small Cell / immunology*
  • Eye Proteins*
  • Female
  • Hippocalcin
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Lipoproteins*
  • Lung Diseases / immunology*
  • Lung Neoplasms / immunology*
  • Male
  • Middle Aged
  • Nerve Tissue Proteins*
  • Recoverin
  • Sensitivity and Specificity


  • Antigens, Neoplasm
  • Autoantibodies
  • Biomarkers, Tumor
  • Calcium-Binding Proteins
  • Eye Proteins
  • Lipoproteins
  • Nerve Tissue Proteins
  • RCVRN protein, human
  • Recoverin
  • Hippocalcin