Heterogeneous nuclear ribonucleoprotein E2 binds to tau exon 10 and moderately activates its splicing

Gene. 2004 Apr 28;331:107-14. doi: 10.1016/j.gene.2004.02.005.

Abstract

Tau is a microtubule-associated protein whose transcript undergoes complex-regulated splicing in the mammalian nervous system. Exon 10 of the gene is an alternatively spliced cassette, which is adult-specific and codes for a microtubule-binding domain. Mutations that affect splicing of exon 10 have been shown to cause frontotemporal dementia with parkinsonism (FTDP). Using tau exon 10 as a bait in a yeast three-hybrid screen, we discovered that it interacts with hnRNPE2. Cotransfection assays show that hnRNPE2 isoforms moderately activate the splicing of exon 10.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Alternative Splicing*
  • Animals
  • Base Sequence
  • Brain / embryology
  • Brain / metabolism
  • COS Cells
  • Cerebellum / metabolism
  • Cerebral Cortex / metabolism
  • Chlorocebus aethiops
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Exons / genetics*
  • Gene Expression
  • Gene Expression Regulation, Developmental
  • Heterogeneous-Nuclear Ribonucleoproteins / genetics
  • Heterogeneous-Nuclear Ribonucleoproteins / metabolism*
  • Humans
  • Molecular Sequence Data
  • Protein Binding
  • Two-Hybrid System Techniques
  • Yeasts / genetics
  • tau Proteins / genetics*

Substances

  • DNA-Binding Proteins
  • Heterogeneous-Nuclear Ribonucleoproteins
  • tau Proteins