Abstract
We have examined mRNA and protein distribution for the axon guidance molecules semaphorin3A, 3F, 4F and semaphorin receptors neuropilin-1 and 2, 1-21 days after intramedullary axotomy of rat lumbar spinal cord motoneurons. We show that semaphorin3A mRNA and protein are up-regulated in the scar and in motoneurons from 3 days and upto 3 weeks after injury. Neuropilin-1 mRNA showed no changed expression in axotomized motoneurons. Semaphorin3F mRNA expression was found in ventral roots after ventral funiculus lesion (VFL) and neuropilin-2 mRNA was found in affected motoneurons from 1 day after injury throughout the examined period. Semaphorin4F mRNA was first found in motoneurons 3 weeks after lesion. These results suggest semaphorin/neuropilin involvement in the injury response of intramedullary axotomized motoneurons.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Axotomy
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Disease Models, Animal
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Female
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Growth Cones / metabolism*
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Growth Cones / ultrastructure
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Motor Neurons / cytology
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Motor Neurons / metabolism*
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Nerve Regeneration / physiology*
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Neuropilin-1 / genetics
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Neuropilin-1 / metabolism
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Neuropilin-2 / genetics
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Neuropilin-2 / metabolism
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Neuropilins / genetics
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Neuropilins / metabolism*
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RNA, Messenger / metabolism
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Rats
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Rats, Sprague-Dawley
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Semaphorin-3A / genetics
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Semaphorin-3A / metabolism
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Semaphorins / genetics
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Semaphorins / metabolism*
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Spinal Cord Injuries / physiopathology
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Spinal Cord Injuries / therapy
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Spinal Nerve Roots / cytology
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Spinal Nerve Roots / growth & development*
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Spinal Nerve Roots / injuries
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Up-Regulation / genetics
Substances
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Membrane Proteins
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Nerve Tissue Proteins
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Neuropilin-2
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Neuropilins
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RNA, Messenger
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Sema3f protein, mouse
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Semaphorin-3A
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Semaphorins
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Neuropilin-1