Semaphorin and neuropilin expression in motoneurons after intraspinal motoneuron axotomy

Neuroreport. 2004 Mar 22;15(4):649-54. doi: 10.1097/00001756-200403220-00015.

Abstract

We have examined mRNA and protein distribution for the axon guidance molecules semaphorin3A, 3F, 4F and semaphorin receptors neuropilin-1 and 2, 1-21 days after intramedullary axotomy of rat lumbar spinal cord motoneurons. We show that semaphorin3A mRNA and protein are up-regulated in the scar and in motoneurons from 3 days and upto 3 weeks after injury. Neuropilin-1 mRNA showed no changed expression in axotomized motoneurons. Semaphorin3F mRNA expression was found in ventral roots after ventral funiculus lesion (VFL) and neuropilin-2 mRNA was found in affected motoneurons from 1 day after injury throughout the examined period. Semaphorin4F mRNA was first found in motoneurons 3 weeks after lesion. These results suggest semaphorin/neuropilin involvement in the injury response of intramedullary axotomized motoneurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axotomy
  • Disease Models, Animal
  • Female
  • Growth Cones / metabolism*
  • Growth Cones / ultrastructure
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism
  • Motor Neurons / cytology
  • Motor Neurons / metabolism*
  • Nerve Regeneration / physiology*
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Neuropilin-1 / genetics
  • Neuropilin-1 / metabolism
  • Neuropilin-2 / genetics
  • Neuropilin-2 / metabolism
  • Neuropilins / genetics
  • Neuropilins / metabolism*
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Semaphorin-3A / genetics
  • Semaphorin-3A / metabolism
  • Semaphorins / genetics
  • Semaphorins / metabolism*
  • Spinal Cord Injuries / physiopathology
  • Spinal Cord Injuries / therapy
  • Spinal Nerve Roots / cytology
  • Spinal Nerve Roots / growth & development*
  • Spinal Nerve Roots / injuries
  • Up-Regulation / genetics

Substances

  • Membrane Proteins
  • Nerve Tissue Proteins
  • Neuropilin-2
  • Neuropilins
  • RNA, Messenger
  • Sema3f protein, mouse
  • Semaphorin-3A
  • Semaphorins
  • Neuropilin-1