Expression of c-Fos and NADPH-d after peripheral noxious stimulation in the pedunculopontine tegmental nucleus

Neuroreport. 2004 Mar 1;15(3):421-3. doi: 10.1097/00001756-200403010-00008.

Abstract

The participation of pedunculopontine tegmental nucleus in descending pain control mechanisms has been proposed. The aim of this study was to investigate NADPH-d and c-Fos reactivity in the pedunculopontine tegmental nucleus following noxious peripheral stimulation. Formalin induced hind paw inflammation in the rat resulted in bilateral increase of Fos and NADPH-d expression in the pedunculopontine tegmental nucleus. Approximately 30% of labelled cells were double-labelled. Close proximity to NADPH-d labelled neuronal processes with Fos-labelled nuclei were observed. This anatomical relationship supports the hypothesis that pedunculopontine tegmental nucleus is involved in pain modulation and proposes a new explanation for the presence of nitric oxide in this nucleus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Formaldehyde
  • Genes, fos / genetics*
  • Immunohistochemistry
  • Male
  • Mesencephalon / metabolism*
  • NADPH Dehydrogenase / biosynthesis*
  • NADPH Dehydrogenase / genetics*
  • Neurons / metabolism
  • Nitric Oxide / physiology*
  • Pain / chemically induced
  • Pain / metabolism*
  • Physical Stimulation
  • Pons / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Tegmentum Mesencephali / metabolism*

Substances

  • Formaldehyde
  • Nitric Oxide
  • NADPH Dehydrogenase