Proliferation of human melanoma cells is under tight control of protein kinase C alpha

J Cell Physiol. 2004 Jun;199(3):381-7. doi: 10.1002/jcp.10434.

Abstract

Exponential proliferation of human melanoma cells has been associated with low levels of protein kinase C (PKC)-alpha. The aim of the present study was to investigate the functional relationship between PKC-alpha and melanoma cell proliferation. Treatment of human melanoma cells with the selective PKC inhibitor Ro-31-8220 resulted in a significant increase of cell proliferation as measured by (3)H-thymidine incorporation and a fluorometric microassay. In addition, phosphorothioate antisense-oligodeoxynucleotides (ODNs) to PKC-alpha enhanced DNA-synthesis of human melanoma cells. Furthermore, microinjection and transient transfection of melanoma cells with PKC-alpha decreased their proliferation, as shown by the reduction of nuclear staining with the proliferation marker Ki-67. The presented data demonstrate a cause-effect relationship between PKC-alpha and melanoma cell growth, whereby PKC-alpha reversely influences the rate of cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division / drug effects
  • Cell Line, Tumor
  • Enzyme Inhibitors / pharmacology
  • Fluorescent Antibody Technique
  • Humans
  • Indoles / pharmacology
  • Melanoma / enzymology*
  • Microinjections
  • Microscopy, Confocal
  • Oligodeoxyribonucleotides, Antisense
  • Protein Kinase C / drug effects
  • Protein Kinase C / metabolism*
  • Protein Kinase C / pharmacology
  • Protein Kinase C-alpha
  • Transfection

Substances

  • Enzyme Inhibitors
  • Indoles
  • Oligodeoxyribonucleotides, Antisense
  • PRKCA protein, human
  • Protein Kinase C
  • Protein Kinase C-alpha
  • Ro 31-8220