Adiponectin specifically increased tissue inhibitor of metalloproteinase-1 through interleukin-10 expression in human macrophages

Circulation. 2004 May 4;109(17):2046-9. doi: 10.1161/01.CIR.0000127953.98131.ED. Epub 2004 Apr 19.


Background: Vascular inflammation and subsequent matrix degradation play an important role in the development of atherosclerosis. We previously reported that adiponectin, an adipose-specific plasma protein, accumulated to the injured artery and attenuated vascular inflammatory response. Clinically, high plasma adiponectin level was associated with low cardiovascular event rate in patients with chronic renal failure. The present study was designed to elucidate the effects of adiponectin on matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases (TIMPs) in human monocyte-derived macrophages.

Methods and results: Human monocyte-derived macrophages were incubated with the physiological concentrations of human recombinant adiponectin for the time indicated. Adiponectin treatment dose-dependently increased TIMP-1 mRNA levels without affecting MMP-9 mRNA levels. Adiponectin also augmented TIMP-1 secretion into the media, whereas MMP-9 secretion and activity were unchanged. Time course experiments indicated that TIMP-1 mRNA levels started to increase at 24 hours of adiponectin treatment and were significantly elevated at 48 hours. Adiponectin significantly increased interleukin-10 (IL-10) mRNA expression at the transcriptional level within 6 hours and significantly increased IL-10 protein secretion within 24 hours. Cotreatment of adiponectin with anti-IL-10 monoclonal antibody completely abolished adiponectin-induced TIMP-1 mRNA expression.

Conclusions: Adiponectin selectively increased TIMP-1 expression in human monocyte-derived macrophages through IL-10 induction. This study identified, for the first time, the adiponectin/IL-10 interaction against vascular inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiponectin
  • Arteriosclerosis / etiology
  • Arteriosclerosis / prevention & control
  • Cells, Cultured / metabolism
  • Gene Expression Regulation / drug effects*
  • Humans
  • Inflammation
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-10 / biosynthesis*
  • Interleukin-10 / genetics
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • Matrix Metalloproteinase 9 / biosynthesis
  • Matrix Metalloproteinase 9 / genetics
  • Matrix Metalloproteinase 9 / metabolism
  • Monocytes / cytology
  • Monocytes / drug effects
  • Paracrine Communication
  • Promoter Regions, Genetic
  • Proteins / pharmacology*
  • Proteins / physiology
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Recombinant Proteins / pharmacology
  • Tissue Inhibitor of Metalloproteinase-1 / biosynthesis*
  • Tissue Inhibitor of Metalloproteinase-1 / genetics
  • Tissue Inhibitor of Metalloproteinase-1 / metabolism
  • Transfection


  • Adiponectin
  • Intercellular Signaling Peptides and Proteins
  • Proteins
  • RNA, Messenger
  • Recombinant Proteins
  • Tissue Inhibitor of Metalloproteinase-1
  • Interleukin-10
  • Matrix Metalloproteinase 9