Identification of GRP 78 (BiP) as a liver cell expressed receptor element for dengue virus serotype 2

Arch Virol. 2004 May;149(5):915-27. doi: 10.1007/s00705-003-0263-x. Epub 2004 Jan 5.


This study sought to identify receptor elements for dengue virus serotype 2 on human liver cells (HepG2) using the viral overlay protein binding assay (VOPBA) technique and Mass Spectrometry fingerprinting. A single major and several minor virus binding bands were observed, and mass spectrometry identified a candidate binding protein for the major binding band as GRP 78 (BiP). GRP78 expression on the cell surface was confirmed, and antibodies directed against both the N and C-terminus of GRP 78 (BiP) altered both the binding of the virus to the cell surface as well as the infectivity profile of HepG2 cells in response to dengue serotype 2 infection. GRP 78 (BiP), which has previously been identified as a co-receptor protein for coxsackievirus A9, is the first non-Fc receptor protein identified for the dengue virus, although GRP78 probably functions as part of a receptor complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / analysis
  • Carrier Proteins / metabolism*
  • Cell Line
  • Dengue Virus / growth & development*
  • Heat-Shock Proteins*
  • Humans
  • Immunoassay
  • Liver / metabolism
  • Liver / virology*
  • Mass Spectrometry
  • Molecular Chaperones*
  • Peptide Mapping
  • Receptors, Virus / analysis
  • Receptors, Virus / metabolism*
  • Viral Plaque Assay


  • Carrier Proteins
  • Heat-Shock Proteins
  • Molecular Chaperones
  • Receptors, Virus
  • dengue virus receptor
  • molecular chaperone GRP78