Regulation of stearoyl-CoA desaturase-1 after central and peripheral nerve lesions

BMC Neurosci. 2004 Apr 20;5:15. doi: 10.1186/1471-2202-5-15.

Abstract

Background: Interruption of mature axons activates a cascade of events in neuronal cell bodies which leads to various outcomes from functional regeneration in the PNS to the failure of any significant regeneration in the CNS. One factor which seems to play an important role in the molecular programs after axotomy is the stearoyl Coenzyme A-desaturase-1 (SCD-1). This enzyme is needed for the conversion of stearate into oleate. Beside its role in membrane synthesis, oleate could act as a neurotrophic factor, involved in signal transduction pathways via activation of protein kinases C.

Results: In situ hybridization and immunohistochemistry demonstrated a strong up-regulation of SCD at mRNA and protein level in regenerating neurons of the rat facial nucleus whereas non-regenerating Clarke's and Red nucleus neurons did not show an induction of this gene.

Conclusion: This differential expression points to a functionally significant role for the SCD-1 in the process of regeneration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Axotomy
  • Central Nervous System / enzymology*
  • Central Nervous System / injuries
  • Central Nervous System / pathology
  • Disease Progression
  • Facial Nerve Injuries / enzymology
  • Facial Nerve Injuries / pathology
  • Hypoglossal Nerve / enzymology
  • Hypoglossal Nerve / pathology
  • Hypoglossal Nerve Injuries
  • Immunohistochemistry
  • In Situ Hybridization
  • Isoenzymes / metabolism
  • Nerve Regeneration / physiology*
  • Neurons / enzymology
  • Neurons / pathology
  • Peripheral Nervous System / enzymology*
  • Peripheral Nervous System / injuries
  • Peripheral Nervous System / pathology
  • Pons / enzymology
  • Pons / pathology
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Red Nucleus / enzymology
  • Red Nucleus / pathology
  • Spinal Cord Injuries / enzymology
  • Spinal Cord Injuries / pathology
  • Stearoyl-CoA Desaturase / metabolism*
  • Trauma, Nervous System / enzymology*
  • Trauma, Nervous System / pathology
  • Up-Regulation

Substances

  • Isoenzymes
  • RNA, Messenger
  • Stearoyl-CoA Desaturase