Proteoglycan and collagen sensitive MRI evaluation of normal and degenerated articular cartilage

J Orthop Res. 2004 May;22(3):557-64. doi: 10.1016/j.orthres.2003.09.008.


Quantitative magnetic resonance imaging (MRI) techniques have earlier been developed to characterize the structure and composition of articular cartilage. Particularly, Gd-DTPA(2-)-enhanced T1 imaging is sensitive to cartilage proteoglycan content, while T2 relaxation time mapping is indicative of the integrity and arrangement of the collagen network. However, the ability of these techniques to detect early osteoarthrotic changes in cartilage has not been demonstrated. In this study, normal and spontaneously degenerated bovine patellar cartilage samples (n=32) were investigated in vitro using the aforementioned techniques. For reference, mechanical, histological and biochemical properties of the adjacent tissue were determined, and a grading system, the cartilage quality index (CQI), was used to score the structural and functional integrity of each sample. As cartilage degeneration progressed, a statistically significant increase in the superficial T2 (r=0.494, p<0.05) and a decrease in superficial and bulk T1 in the presence of Gd-DTPA(2-) (r=-0.681 and -0.688 (p<0.05), respectively) were observed. Gd-DTPA(2-)-enhanced T1 imaging served as the best predictor of tissue integrity and accounted for about 50% of the variation in CQI. The present results reveal that changes in the quantitative MRI parameters studied are indicative of structural and compositional alterations as well as the mechanical impairment of spontaneously degenerated articular cartilage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cartilage, Articular / pathology*
  • Cartilage, Articular / physiopathology
  • Cattle
  • Collagen / analysis*
  • Gadolinium DTPA*
  • Magnetic Resonance Imaging*
  • Osteoarthritis / pathology*
  • Osteoarthritis / physiopathology
  • Proteoglycans / analysis*


  • Proteoglycans
  • Collagen
  • Gadolinium DTPA