B7+ T cells in myeloma: an acquired marker of prior chronic antigen presentation

Leuk Lymphoma. 2004 Feb;45(2):363-71. doi: 10.1080/10428190310001607142.


Murine T cells do not endogenously upregulate CD80 expression but rather acquire CD80 from antigen presenting cells (APC) during CD28 ligation. Murine CD80+ memory T cells undergo apoptosis in the presence of high levels of antigen while naive CD80+ T cells are capable of acting as APC and T cell:T cell ligation induces anergy and unresponsiveness to antigen rechallenge. Reversing T cell unresponsiveness may be a key factor in the development of immunotherapy strategies for patients with myeloma. We have determined that B7+ T cells (CD80+ or CD86+) are common in patients with myeloma (n = 45), can be either CD4 or CD8, tend to be associated with stable disease and are polyclonal memory T cells (CD45RO). CD80 mRNA expression was present in CD80+ monocytes but not in CD3+ cells with a similar level of CD80 antigen expression. CD80 and CD86 antigen expression was upregulated on B cells but not T cells during incubation with trimeric human CD40 ligand (huCD40LT) + IL-2. Although there was a gradual loss of expression during in vitro culture, CD80+ T cells could be purified for further study. We conclude that B7 expression is common on T cells of patients with myeloma but that this is acquired rather than endogenously produced. B7+ CD45RO+ T cells constitute a population of memory T cells chronically exposed to antigen and warrant further study.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation*
  • Antigens, CD / biosynthesis
  • Antigens, Differentiation / biosynthesis
  • Apoptosis
  • B7-1 Antigen / biosynthesis*
  • B7-2 Antigen
  • CD28 Antigens / biosynthesis
  • CD3 Complex / biosynthesis
  • CD4-Positive T-Lymphocytes / metabolism
  • CD8-Positive T-Lymphocytes / metabolism
  • CTLA-4 Antigen
  • Cell Separation
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • Flow Cytometry
  • Humans
  • Immunologic Memory
  • Immunotherapy / methods
  • Leukocyte Common Antigens / biosynthesis
  • Membrane Glycoproteins / biosynthesis
  • Mice
  • Multiple Myeloma / blood*
  • Phenotype
  • Polymerase Chain Reaction
  • RNA, Messenger / metabolism
  • Receptors, Antigen, T-Cell, alpha-beta / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes / metabolism*
  • Up-Regulation


  • Antigens, CD
  • Antigens, Differentiation
  • B7-1 Antigen
  • B7-2 Antigen
  • CD28 Antigens
  • CD3 Complex
  • CD86 protein, human
  • CTLA-4 Antigen
  • CTLA4 protein, human
  • Cd86 protein, mouse
  • Ctla4 protein, mouse
  • DNA, Complementary
  • Membrane Glycoproteins
  • RNA, Messenger
  • Receptors, Antigen, T-Cell, alpha-beta
  • Leukocyte Common Antigens