Abstract
We have previously demonstrated that activin, a member of the TGF-beta family, has a potent mesoderm-inducing activity in Xenopus embryos. In the course of screening for activin-related genes from Xenopus, we have cloned cDNAs for Xenopus homologue of BMP-2, -4 and -7. Northern blot analysis revealed that these BMP genes are maternally encoded and differentially regulated after fertilization. Alkaline phosphatase-inducing assay using the recombinant BMP proteins has shown that at least BMP-2 and -4 have similar activity to mammalian counterparts.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / pharmacology
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Amino Acid Sequence
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Animals
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Blotting, Northern
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Bone Morphogenetic Proteins
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Cell Line
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DNA / genetics
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DNA / isolation & purification
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Embryo, Nonmammalian / physiology*
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Gene Expression Regulation*
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Growth Substances / genetics*
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Molecular Sequence Data
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Proteins / genetics*
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Proteins / pharmacology
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RNA, Messenger / genetics
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RNA, Messenger / isolation & purification
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Recombinant Proteins / pharmacology
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Restriction Mapping
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Sequence Homology, Nucleic Acid
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Software
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Transcription, Genetic*
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Transfection
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Xenopus / embryology
Substances
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Bone Morphogenetic Proteins
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Growth Substances
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Proteins
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RNA, Messenger
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Recombinant Proteins
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DNA
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Alkaline Phosphatase
Associated data
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GENBANK/D10916
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GENBANK/D10917
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GENBANK/D10918
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GENBANK/D10919
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GENBANK/D10920
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GENBANK/S39221
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GENBANK/X63424
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GENBANK/X63425
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GENBANK/X63426
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GENBANK/X63427