Genes for bone morphogenetic proteins are differentially transcribed in early amphibian embryos

Biochem Biophys Res Commun. 1992 Aug 14;186(3):1487-95. doi: 10.1016/s0006-291x(05)81574-8.

Abstract

We have previously demonstrated that activin, a member of the TGF-beta family, has a potent mesoderm-inducing activity in Xenopus embryos. In the course of screening for activin-related genes from Xenopus, we have cloned cDNAs for Xenopus homologue of BMP-2, -4 and -7. Northern blot analysis revealed that these BMP genes are maternally encoded and differentially regulated after fertilization. Alkaline phosphatase-inducing assay using the recombinant BMP proteins has shown that at least BMP-2 and -4 have similar activity to mammalian counterparts.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase / pharmacology
  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Bone Morphogenetic Proteins
  • Cell Line
  • DNA / genetics
  • DNA / isolation & purification
  • Embryo, Nonmammalian / physiology*
  • Gene Expression Regulation*
  • Growth Substances / genetics*
  • Molecular Sequence Data
  • Proteins / genetics*
  • Proteins / pharmacology
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Recombinant Proteins / pharmacology
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Software
  • Transcription, Genetic*
  • Transfection
  • Xenopus / embryology

Substances

  • Bone Morphogenetic Proteins
  • Growth Substances
  • Proteins
  • RNA, Messenger
  • Recombinant Proteins
  • DNA
  • Alkaline Phosphatase

Associated data

  • GENBANK/D10916
  • GENBANK/D10917
  • GENBANK/D10918
  • GENBANK/D10919
  • GENBANK/D10920
  • GENBANK/S39221
  • GENBANK/X63424
  • GENBANK/X63425
  • GENBANK/X63426
  • GENBANK/X63427