Coronaviridae and SARS-associated coronavirus strain HSR1

Emerg Infect Dis. 2004 Mar;10(3):413-8. doi: 10.3201/eid1003.030683.

Abstract

During the recent severe acute respiratory (SARS) outbreak, the etiologic agent was identified as a new coronavirus (CoV). We have isolated a SARS-associated CoV (SARS-CoV) strain by injecting Vero cells with a sputum specimen from an Italian patient affected by a severe pneumonia; the patient traveled from Vietnam to Italy in March 2003. Ultrastructural analysis of infected Vero cells showed the virions within cell vesicles and around the cell membrane. The full-length viral genome sequence was similar to those derived from the Hong-Kong Hotel M isolate. By using both real-time reverse transcription-polymerase chain reaction TaqMan assay and an infectivity plaque assay, we determined that approximately 360 viral genomes were required to generate a PFU. In addition, heparin (100 microg/mL) inhibited infection of Vero cells by 50%. Overall, the molecular and biologic characteristics of the strain HSR1 provide evidence that SARS-CoV forms a fourth genetic coronavirus group with distinct genomic and biologic features.

MeSH terms

  • Animals
  • Chlorocebus aethiops
  • Communicable Diseases, Emerging / virology*
  • Genome, Viral*
  • Humans
  • Male
  • Reverse Transcriptase Polymerase Chain Reaction
  • SARS Virus / genetics*
  • SARS Virus / isolation & purification
  • SARS Virus / pathogenicity
  • Severe Acute Respiratory Syndrome / virology*
  • Sputum / virology
  • Vero Cells