Immunofluorescence assay for serologic diagnosis of SARS

Emerg Infect Dis. 2004 Mar;10(3):530-2. doi: 10.3201/eid1003.030493.


We evaluated an indirect immunofluorescence assay based on virus-infected cells for detecting anti-severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Viral / isolation & purification
  • Child
  • Child, Preschool
  • Communicable Diseases, Emerging / diagnosis*
  • Communicable Diseases, Emerging / immunology
  • Communicable Diseases, Emerging / physiopathology
  • Female
  • Fluorescent Antibody Technique, Indirect / methods*
  • Hong Kong
  • Humans
  • Male
  • Middle Aged
  • Reverse Transcriptase Polymerase Chain Reaction
  • Severe Acute Respiratory Syndrome / diagnosis*
  • Severe Acute Respiratory Syndrome / immunology
  • Severe Acute Respiratory Syndrome / physiopathology


  • Antibodies, Viral