Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation

Shuji Takeda; Shinya Tsukiji; Teruyuki Nagamune

doi:10.1016/j.bmcl.2004.03.023

Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation

Bioorg Med Chem Lett. 2004 May 17;14(10):2407-10. doi: 10.1016/j.bmcl.2004.03.023.

Authors

Shuji Takeda¹, Shinya Tsukiji, Teruyuki Nagamune

Affiliation

¹ Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan.

PMID: 15109622
DOI: 10.1016/j.bmcl.2004.03.023

Abstract

We developed a convenient method for synthesizing homogeneous DNA-protein conjugates. The method is based on expressed protein ligation of intein-fusion proteins and oligonucleotides derivatized with a cysteine. A range of cysteinyl oligonucleotides were synthesized by using a new reagent 1 and were successfully applied to expressed protein ligation to attach the oligonucleotides specifically at the C-terminus of a recombinant protein.

MeSH terms

Amino Acid Sequence
Cross-Linking Reagents
Cysteine / chemistry
Inteins
Oligodeoxyribonucleotides / chemistry*
Recombinant Proteins / chemistry*

Substances

Cross-Linking Reagents
Oligodeoxyribonucleotides
Recombinant Proteins
Cysteine