Site-specific conjugation of oligonucleotides to the C-terminus of recombinant protein by expressed protein ligation

Bioorg Med Chem Lett. 2004 May 17;14(10):2407-10. doi: 10.1016/j.bmcl.2004.03.023.


We developed a convenient method for synthesizing homogeneous DNA-protein conjugates. The method is based on expressed protein ligation of intein-fusion proteins and oligonucleotides derivatized with a cysteine. A range of cysteinyl oligonucleotides were synthesized by using a new reagent 1 and were successfully applied to expressed protein ligation to attach the oligonucleotides specifically at the C-terminus of a recombinant protein.

MeSH terms

  • Amino Acid Sequence
  • Cross-Linking Reagents
  • Cysteine / chemistry
  • Inteins
  • Oligodeoxyribonucleotides / chemistry*
  • Recombinant Proteins / chemistry*


  • Cross-Linking Reagents
  • Oligodeoxyribonucleotides
  • Recombinant Proteins
  • Cysteine