A phage with high affinity for hepatitis B surface antigen for the detection of HBsAg

Xuanyong Lu; Paul Weiss; Timothy Block

doi:10.1016/j.jviromet.2004.02.014

A phage with high affinity for hepatitis B surface antigen for the detection of HBsAg

J Virol Methods. 2004 Jul;119(1):51-4. doi: 10.1016/j.jviromet.2004.02.014.

Authors

Xuanyong Lu¹, Paul Weiss, Timothy Block

Affiliation

¹ Jefferson Center for Biomedical Research and Agricultural Medicine, Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, 700 East Butler Avenue, Doylestown, PA 18901, USA. xuanyong.lu@jefferson.edu

PMID: 15109821
DOI: 10.1016/j.jviromet.2004.02.014

Abstract

An M13 phage, called PHH2, with the ability to bind HBsAg was isolated from a coat protein III display library. The region of the HBsAg polypeptide to which PHH2 binds was determined. The HBsAg binding phage was used in an assay referred to as "PHALISA", an abbreviation for phage-linked immune-absorbent assay. This assay was at least 20-100 times more sensitive in the detection of HBV antigen than conventional enzyme-linked immune-absorbent assays (ELISA). The application of this method for screening and detection of specific protein is discussed.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Bacteriophage M13 / genetics
Base Sequence
DNA Primers / genetics
Enzyme-Linked Immunosorbent Assay
Hepatitis B Surface Antigens / genetics
Hepatitis B Surface Antigens / immunology
Hepatitis B Surface Antigens / isolation & purification*
Peptide Library
Sensitivity and Specificity
Virology / methods
Virology / statistics & numerical data

Substances

DNA Primers
Hepatitis B Surface Antigens
Peptide Library