Insulin receptor splicing alteration in myotonic dystrophy type 2

Am J Hum Genet. 2004 Jun;74(6):1309-13. doi: 10.1086/421528. Epub 2004 Apr 26.


Myotonic dystrophy (DM) is caused by either an untranslated CTG expansion in the 3' untranslated region of the DMPK gene on chromosome 19 (dystrophia myotonica type 1 [DM1]), or an untranslated CCTG tetranucleotide repeat expansion in intron 1 of the ZNF9 gene on chromosome 3 (dystrophia myotonica type 2 [DM2]). RNA-binding proteins adhere to transcripts of the repeat expansions that accumulate in the nucleus, and a trans-dominant dysregulation of pre-mRNA alternative splicing has been demonstrated for several genes. In muscle from patients with DM1, altered insulin-receptor splicing to the nonmuscle isoform corresponds to the insulin insensitivity and diabetes that are part of the DM phenotype; because of insulin-receptor species differences, this effect is not seen in mouse models of the disease. We now demonstrate that comparable splicing abnormalities occur in DM2 muscle prior to the development of muscle histopathology, thus demonstrating an early pathogenic effect of RNA expansions.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Aged
  • Alternative Splicing / genetics*
  • Case-Control Studies
  • Female
  • Glucose / metabolism
  • Humans
  • In Situ Hybridization, Fluorescence
  • Insulin Resistance / genetics*
  • Male
  • Middle Aged
  • Myotonic Dystrophy / diagnosis
  • Myotonic Dystrophy / genetics*
  • Myotonic Dystrophy / pathology
  • RNA Probes
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptor, Insulin / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction


  • RNA Probes
  • RNA, Messenger
  • Receptor, Insulin
  • Glucose