A gene encoding a superoxide dismutase of the facultative intracellular bacterium Listeria monocytogenes

Gene. 1992 Sep 1;118(1):121-5. doi: 10.1016/0378-1119(92)90258-q.

Abstract

A gene (lmsod) encoding superoxide dismutase (SOD; EC 1.15.1.1) of the facultative intracellular pathogen, Listeria monocytogenes, was cloned by functional complementation of an SOD-deficient Escherichia coli mutant. The nucleotide sequence was determined and the deduced amino acid (aa) sequence (202 aa) showed close similarity to manganese-containing SOD's from other organisms. Subunits of the recombinant L. monocytogenes SOD (re-SOD) and of both E. coli SODs formed enzymatically active hybrid enzymes in vivo. DNA/DNA-hybridization experiments showed that this type of recombinant re-sod gene is conserved within the genus Listeria.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Genes, Bacterial / genetics*
  • Genetic Complementation Test
  • Listeria monocytogenes / enzymology
  • Listeria monocytogenes / genetics*
  • Manganese
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Sequence Homology, Nucleic Acid
  • Superoxide Dismutase / genetics*

Substances

  • DNA, Bacterial
  • Manganese
  • Superoxide Dismutase

Associated data

  • GENBANK/M74891
  • GENBANK/M74892
  • GENBANK/M79309
  • GENBANK/M79310
  • GENBANK/M80526
  • GENBANK/M88536
  • GENBANK/M88537
  • GENBANK/M88538
  • GENBANK/M88539
  • GENBANK/M90357