An improved suicide vector for construction of chromosomal insertion mutations in bacteria

Gene. 1992 Sep 1;118(1):145-6. doi: 10.1016/0378-1119(92)90263-o.


We have constructed an R6K-based suicide vector (pJP5603) that requires a trans supply of the pir-encoded pi protein of plasmid R6K for replication. Therefore, efficient plasmid suicide results upon transfer to bacteria not harbouring pir. The 3.1-kb vector encodes kanamycin resistance and is mobilizable. When used in conjunction with a JM109 strain carrying pir, it has nine unique restriction sites available for alpha-complementation cloning. Vector functionality was demonstrated in Rhodobacter sphaeroides.

MeSH terms

  • Bacterial Proteins / genetics*
  • Chromosomes, Bacterial
  • DNA Helicases*
  • DNA, Recombinant / genetics
  • DNA-Binding Proteins*
  • Genetic Vectors / genetics*
  • Mutagenesis, Insertional / methods*
  • Replicon / genetics
  • Rhodobacter sphaeroides / genetics*
  • Trans-Activators*


  • Bacterial Proteins
  • DNA, Recombinant
  • DNA-Binding Proteins
  • Trans-Activators
  • replication initiator protein
  • DNA Helicases