A new inhibitor of metalloproteinases from chicken: ChIMP-3. A third member of the TIMP family

J Biol Chem. 1992 Aug 25;267(24):17321-6.


We report cDNA cloning and primary structure of a new metalloproteinase inhibitor (ChIMP-3) produced by chicken embryo fibroblasts. ChIMP-3, formerly called the 21-kDa protein, is one of five ChIMPs (Chicken Inhibitor of MetalloProteinases). In this paper, we report that of the three most abundant ChIMPs, ChIMP-3 and ChIMP-a are extracellular matrix components, whereas ChIMP-2 is found in the media conditioned by the cells. Treatment of ChIMP-3 and ChIMP-a with N-glycosidase-F indicates that ChIMP-a is N-glycosylated whereas ChIMP-3 is not. The deduced amino acid sequence of ChIMP-3 predicts a protein whose properties are consistent with experimental measurements. Analysis of sequence alignments with the two previously described members of the TIMP (tissue inhibitor of metalloproteinases) family, TIMP-1 and TIMP-2, from various species indicates that ChIMP-3 is a related but distinct protein. This conclusion is supported by lack of significant binding with anti-TIMP-1 and anti-TIMP-2 antibodies. Based on these data, its unusual solubility properties, and its exclusive location in the matrix, we propose that ChIMP-3 is a new member of this family of metalloproteinase inhibitors, a TIMP-3.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Chick Embryo
  • Cloning, Molecular
  • DNA / genetics*
  • DNA / isolation & purification
  • Glycoproteins / genetics*
  • Glycoside Hydrolases
  • Glycosylation
  • Metalloendopeptidases / antagonists & inhibitors*
  • Metalloendopeptidases / genetics
  • Metalloendopeptidases / isolation & purification
  • Molecular Sequence Data
  • Multigene Family*
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction
  • Proteins / genetics*
  • Proteins / isolation & purification
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Software
  • Tissue Inhibitor of Metalloproteinase-3
  • Tissue Inhibitor of Metalloproteinases


  • Glycoproteins
  • Oligodeoxyribonucleotides
  • Proteins
  • Tissue Inhibitor of Metalloproteinase-3
  • Tissue Inhibitor of Metalloproteinases
  • DNA
  • Glycoside Hydrolases
  • Metalloendopeptidases

Associated data

  • GENBANK/M84424
  • GENBANK/M92918
  • GENBANK/M92919
  • GENBANK/M92920
  • GENBANK/M93702
  • GENBANK/M93703
  • GENBANK/M93704
  • GENBANK/M93705
  • GENBANK/M94144
  • GENBANK/M94531