Functional expression of the ectoine hydroxylase gene (thpD) from Streptomyces chrysomallus in Halomonas elongata

Appl Environ Microbiol. 2004 May;70(5):3130-2. doi: 10.1128/aem.70.5.3130-3132.2004.

Abstract

The formation of hydroxyectoine in the industrial ectoine producer Halomonas elongata was improved by the heterologous expression of the ectoine hydroxylase gene, thpD, from Streptomyces chrysomallus. The efficient conversion of ectoine to hydroxyectoine was achieved by the concerted regulation of thpD by the H. elongata ectA promoter.

MeSH terms

  • Amino Acids, Diamino / biosynthesis
  • Amino Acids, Diamino / metabolism*
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Biotechnology / methods
  • Gene Expression Regulation, Bacterial
  • Halomonas / enzymology*
  • Halomonas / genetics
  • Mixed Function Oxygenases / genetics*
  • Mixed Function Oxygenases / metabolism*
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Streptomyces / enzymology*
  • Streptomyces / genetics

Substances

  • Amino Acids, Diamino
  • Bacterial Proteins
  • hydroxyectoine
  • ectoine
  • Mixed Function Oxygenases

Associated data

  • GENBANK/AY524544