Abstract
Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increase folate levels in many fermented food products.
MeSH terms
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Biotechnology / methods
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Cloning, Molecular
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DNA, Bacterial / genetics
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Folic Acid / biosynthesis*
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Folic Acid / metabolism
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Gene Expression Regulation, Bacterial
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Lactobacillus / genetics
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Lactobacillus / metabolism*
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Lactococcus lactis / genetics
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Lactococcus lactis / metabolism*
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Multigene Family
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Plasmids
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Transformation, Bacterial*
Substances
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Bacterial Proteins
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DNA, Bacterial
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Folic Acid