Transformation of folate-consuming Lactobacillus gasseri into a folate producer

Appl Environ Microbiol. 2004 May;70(5):3146-8. doi: 10.1128/AEM.70.5.3146-3148.2004.

Abstract

Five genes essential for folate biosynthesis in Lactococcus lactis were cloned on a broad-host-range lactococcal vector and were transferred to the folate auxotroph Lactobacillus gasseri. As a result L. gasseri changed from a folate consumer to a folate producer. This principle can be used to increase folate levels in many fermented food products.

Publication types

  • Evaluation Study

MeSH terms

  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Biotechnology / methods
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • Folic Acid / biosynthesis*
  • Folic Acid / metabolism
  • Gene Expression Regulation, Bacterial
  • Lactobacillus / genetics
  • Lactobacillus / metabolism*
  • Lactococcus lactis / genetics
  • Lactococcus lactis / metabolism*
  • Multigene Family
  • Plasmids
  • Transformation, Bacterial*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Folic Acid