In vivo spin trapping of nitric oxide

Antioxid Redox Signal. 2004 Jun;6(3):649-56. doi: 10.1089/152308604773934413.


The measurement of nitric oxide (NO) in biological samples has normally required destructive chemical techniques. The ability to detect NO non-invasively in living animals or excised organs has great potential using specialized electron paramagnetic resonance (EPR) methods. Although NO is paramagnetic, it cannot be observed directly unless it is complexed with ferrous iron-dithiocarbamate ligand spin trap complexes. Despite the minimally invasive nature of the technique, highly sensitive localized concentrations of NO may be observed ("trapped") in vivo by both L-band EPR and magnetic resonance imaging.

Publication types

  • Review

MeSH terms

  • Animals
  • Brain / pathology
  • Electron Spin Resonance Spectroscopy
  • Ferrous Compounds / chemistry*
  • Lipopolysaccharides / chemistry
  • Liver / metabolism
  • Magnetics
  • Models, Chemical
  • Nitric Oxide / chemistry*
  • Rats
  • Rats, Wistar
  • Spin Labels
  • Spin Trapping / methods*
  • Thiocarbamates / chemistry*
  • Time Factors
  • omega-N-Methylarginine / chemistry


  • Ferrous Compounds
  • Lipopolysaccharides
  • Spin Labels
  • Thiocarbamates
  • dithiocarbamate-Fe(II)
  • omega-N-Methylarginine
  • Nitric Oxide