Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts

FEBS Lett. 2004 May 7;565(1-3):101-5. doi: 10.1016/j.febslet.2004.03.080.

Abstract

An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl(2) was necessary for the protein's activity, whilst NaF inhibited it. The K(m) for pyrophosphate and the pH optimum of the protein was determined. The gene coding for this protein was expressed in all tissues, and its expression in rosette leaves was induced by incubation on metabolizable sugars. In vitro import experiments demonstrated that the protein could be imported into chloroplasts and localized to the stromal compartment.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology*
  • Arabidopsis Proteins / biosynthesis*
  • Arabidopsis Proteins / chemistry*
  • Arabidopsis Proteins / genetics
  • Chloroplasts / enzymology*
  • Chloroplasts / metabolism
  • DNA, Complementary / metabolism
  • Escherichia coli / metabolism
  • Genetic Complementation Test
  • Hydrogen-Ion Concentration
  • Inorganic Pyrophosphatase / biosynthesis*
  • Inorganic Pyrophosphatase / chemistry*
  • Inorganic Pyrophosphatase / genetics
  • Inorganic Pyrophosphatase / metabolism
  • Kinetics
  • Magnesium Chloride / pharmacology
  • Molecular Sequence Data
  • Peas / enzymology
  • Phylogeny
  • Plastids / metabolism
  • Protein Isoforms
  • Protein Transport
  • RNA / chemistry
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Sodium Fluoride / pharmacology
  • Tissue Distribution

Substances

  • Arabidopsis Proteins
  • DNA, Complementary
  • Protein Isoforms
  • RNA, Messenger
  • Magnesium Chloride
  • RNA
  • Sodium Fluoride
  • Inorganic Pyrophosphatase
  • PPa6 protein, Arabidopsis