Cdc14 and condensin control the dissolution of cohesin-independent chromosome linkages at repeated DNA

Cell. 2004 May 14;117(4):455-69. doi: 10.1016/s0092-8674(04)00413-1.

Abstract

Chromosome segregation is triggered by the cleavage of cohesins by separase. Here we show that in budding yeast separation of the ribosomal DNA (rDNA) and telomeres also requires Cdc14, a protein phosphatase known for its role in mitotic exit. Cdc14 shares this role with the FEAR network, which activates Cdc14 during early anaphase, but not the mitotic exit network, which promotes Cdc14 activity during late anaphase. We further show that CDC14 is necessary and sufficient to promote condensin enrichment at the rDNA locus and to trigger rDNA segregation in a condensin-dependent manner. We propose that Cdc14 released by the FEAR network mediates the partitioning of rDNA by facilitating the localization of condensin thereto. This dual role of the FEAR network in initiating mitotic exit and promoting chromosome segregation ensures that exit from mitosis is coupled to the completion of chromosome segregation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / genetics*
  • Adenosine Triphosphatases / metabolism
  • Cell Cycle Proteins / genetics*
  • Cell Cycle Proteins / metabolism
  • Cell Nucleolus / genetics
  • Cell Survival / genetics
  • Chromosomal Proteins, Non-Histone
  • Chromosome Segregation / genetics*
  • DNA, Ribosomal / genetics*
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Endopeptidases / genetics
  • Endopeptidases / metabolism
  • Fungal Proteins
  • Mitosis / genetics
  • Multiprotein Complexes
  • Mutation / genetics
  • Nuclear Proteins / genetics
  • Protein Tyrosine Phosphatases / genetics*
  • Protein Tyrosine Phosphatases / metabolism
  • Repetitive Sequences, Nucleic Acid / genetics
  • SUMO-1 Protein / metabolism
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Saccharomyces cerevisiae Proteins / metabolism
  • Telomere / genetics

Substances

  • CDC14 protein, S cerevisiae
  • Cell Cycle Proteins
  • Chromosomal Proteins, Non-Histone
  • DNA, Ribosomal
  • DNA-Binding Proteins
  • Fungal Proteins
  • Multiprotein Complexes
  • Nuclear Proteins
  • SUMO-1 Protein
  • Saccharomyces cerevisiae Proteins
  • cohesins
  • condensin complexes
  • Protein Tyrosine Phosphatases
  • Endopeptidases
  • ULP2 protein, S cerevisiae
  • Adenosine Triphosphatases