Expression of TAP73 and DeltaNP73 in malignant gliomas

Oncol Rep. 2004 Jun;11(6):1337-41.


The p73 gene is able to encode transcriptionaly active TAp73, as well as a dominant-negatively acting DeltaNp73 transcript isoforms. We studied differential expression of these forms in normal brain as well as glial tumors, by semiquantitative RT-PCR. The expression of p73 was low or undetectable in normal brain tissues. Most of the tumors and non-tumor brain tissues also lacked significant expression of p73 in patients with low-grade astrocytomas. In contrast, most high-grade glial tumors displayed strong up-regulation of TAp73, whereas only a few displayed DeltaNp73 expression. These aberrations may reflect the inactivation of retinoblastoma pathway in these tumors which result in the activation of E2F transcription factors, since TAp73 is a known target of E2F1 gene. The study of TAp73 expression in brain tumors may serve as a means to evaluate the retinoblastoma pathway-dependent tumor progression.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brain / metabolism
  • Brain / pathology
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Gene Expression Regulation, Neoplastic*
  • Genes, Dominant*
  • Genes, Tumor Suppressor
  • Glioma / genetics*
  • Humans
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism*
  • RNA, Neoplasm / genetics
  • RNA, Neoplasm / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Deletion
  • Tumor Protein p73
  • Tumor Suppressor Proteins
  • Up-Regulation


  • DNA-Binding Proteins
  • Nuclear Proteins
  • RNA, Neoplasm
  • TP73 protein, human
  • Tumor Protein p73
  • Tumor Suppressor Proteins
  • delta Np73 protein, human