Effect of phytate on element bioavailability in the second generation of rats

J Trace Elem Med Biol. 2004;17(4):229-34. doi: 10.1016/S0946-672X(04)80023-3.


In this paper the relation between long term consumption of a high dose of sodium phytate and the mineral status of the organism is evaluated in rats. For this purpose, element concentrations (Ca, Mg, Fe, Zn, Mn) were determined in liver, heart, testicle, bone and urine of a second generation of Wistar rats, treated with a phytate free diet (AIN-76A) and with the same diet plus 1% phytate as sodium salt. The most significant differences were observed between bone zinc contents of male and female rats. The zinc content of rats fed a 1% phytate as sodium salt diet resulted clearly lower than that found in no-phytate treated rats. Hence, it is concluded that when up to 1% of phytate as sodium salt is consumed together with an equilibrated purified diet (free of phytate), no decrease in mineral bioavailability is observed in second generation rats, except for an indication of lower zinc availability by lower zinc concentrations in some organs, mainly bone. However, using this purified diet, the zinc concentration in bone resulted around 10 times higher than found in rats fed with a common non purified rat chow.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Availability
  • Bone and Bones / chemistry
  • Diet*
  • Female
  • Liver / chemistry
  • Male
  • Metals / chemistry*
  • Metals / metabolism
  • Myocardium / chemistry
  • Phytic Acid / pharmacokinetics*
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Testis / chemistry
  • Trace Elements / analysis*
  • Urine / chemistry


  • Metals
  • Trace Elements
  • Phytic Acid