Mass spectral analysis in proteomics

Annu Rev Biophys Biomol Struct. 2004;33:297-316. doi: 10.1146/annurev.biophys.33.111502.082538.

Abstract

Mass spectrometry provides key tools for the analysis of proteins. New types of mass spectrometers that provide enhanced capability to discover protein identities and perform improved proteomic experiments are discussed. Handling the complex mixtures of peptides and proteins generated from protein complexes and whole cells requires multidimensional separations; several forms of separation are discussed. Applications of mass spectrometry-based approaches for contemporary proteomic analyses are described.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Chromatography / methods*
  • Chromatography / trends
  • Complex Mixtures / analysis
  • Complex Mixtures / chemistry
  • Electrophoresis / methods*
  • Mass Spectrometry / methods*
  • Mass Spectrometry / trends
  • Proteins / analysis*
  • Proteins / chemistry*
  • Proteome / analysis*
  • Proteome / chemistry*
  • Proteomics / methods*
  • Proteomics / trends

Substances

  • Complex Mixtures
  • Proteins
  • Proteome