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, 23 (1-2), 26-31

Somatic Embryogenesis and Plant Regeneration From Immature Zygotic Embryos of Hinoki Cypress (Chamaecyparis Obtusa Sieb. Et Zucc.)

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Somatic Embryogenesis and Plant Regeneration From Immature Zygotic Embryos of Hinoki Cypress (Chamaecyparis Obtusa Sieb. Et Zucc.)

T Taniguchi et al. Plant Cell Rep.

Abstract

We established a plant regeneration system for Hinoki cypress (Chamaecyparis obtusa) via somatic embryogenesis. Embryogenic tissues were successfully induced on three kinds of Smith media from megagametophyte explants containing pre-cotyledonary embryos of C. obtusa plus-trees. Factors affecting somatic embryo maturation were examined. The concentration of polyethylene glycol 4000 in the medium was a critical factor for embryo maturation and its effective concentration was 150 g/l. The addition of 30 g/l maltose to the medium had a positive effect on embryo maturation, but sucrose was ineffective. The mature somatic embryos germinated at a germination frequency of approximately 60%, and the presence of activated charcoal was effective in stimulating plantlet growth. The plantlets acclimatized successfully in a greenhouse. To our knowledge, this is first report describing details of a plant regeneration method for C. obtusa via somatic embryogenesis.

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Cited by 3 PubMed Central articles

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