Transcriptional Analysis of the groE and dnaK Heat-Shock Operons of Enterococcus Faecalis

Res Microbiol. 2004 May;155(4):252-8. doi: 10.1016/j.resmic.2004.02.002.

Abstract

Enterococcus faecalis is able to survive in extremely adverse conditions, and its ability to resist stress is considered a key virulence attribute. Here, we conducted a detailed transcriptional analysis of the groE and dnaK operons of E. faecalis. The dnaK operon is comprised of four genes (hrcA-grpE-dnaK-dnaJ) preceded by two conserved CIRCE sequences. The dnaK operon is expressed from a sigmaA-type promoter located upstream of hrcA and multiple transcripts are detectable, possibly due to mRNA processing. The groE operon (groES-groEL) is transcribed as a single mRNA from a sigmaA-type promoter located immediately upstream of a CIRCE element. Induction of dnaK and groEL occurs in response to heat shock and exposure to NaCl, SDS and H(2)O(2).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Base Sequence
  • Chaperonins
  • Conserved Sequence
  • DNA, Bacterial / analysis
  • Enterococcus faecalis / genetics*
  • Enterococcus faecalis / metabolism
  • Escherichia coli Proteins
  • Heat-Shock Proteins / genetics*
  • Molecular Sequence Data
  • Operon / genetics*
  • Promoter Regions, Genetic
  • Sodium Chloride / pharmacology
  • Transcription, Genetic*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Escherichia coli Proteins
  • GroE protein, E coli
  • Heat-Shock Proteins
  • Sodium Chloride
  • Chaperonins