Proposal to Accommodate Burkholderia Cepacia Genomovar VI as Burkholderia Dolosa Sp. Nov

Int J Syst Evol Microbiol. 2004 May;54(Pt 3):689-691. doi: 10.1099/ijs.0.02888-0.

Abstract

Phenotypic and genotypic studies revealed new tools for differentiating Burkholderia cepacia genomovar VI from Burkholderia multivorans and other B. cepacia-complex species. Hence, the name Burkholderia dolosa sp. nov. is proposed, with LMG 18943(T) (=CCUG 47727(T)) as the type strain. B. dolosa can be differentiated from other B. cepacia-complex bacteria by its inability to assimilate tryptamine, azelaic acid and salicin and by its failure to grow on the B. cepacia-selective medium PCAT. Both 16S rDNA and recA RFLP analysis revealed unique B. dolosa restriction patterns. In addition, new 16S rDNA- and recA-based PCR assays allowed its specific identification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Burkholderia / classification*
  • Burkholderia / genetics
  • Burkholderia / metabolism
  • Burkholderia cepacia / classification*
  • Burkholderia cepacia / genetics
  • Burkholderia cepacia / metabolism
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Genes, Bacterial
  • Molecular Sequence Data
  • Phenotype
  • Phylogeny
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Rec A Recombinases / genetics

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Rec A Recombinases

Associated data

  • GENBANK/AY324807
  • GENBANK/AY324808