Effect of linoleic acid on proliferation and gene expression in the breast cancer cell line T47D

Cancer Lett. 2004 Jun 8;209(1):25-35. doi: 10.1016/j.canlet.2003.12.010.

Abstract

Human and animal studies have linked n-6 polyunsaturated fatty acids with mammary carcinogenesis. We investigated the cellular and molecular effects of linoleic acid on the human breast cancer cell line T47D. Linoleic acid had a stimulatory effect on the growth of T47D cells, associated with an increase in the proportion of cells in the S phase of the cell cycle. Microarray, functional group and quantitative PCR analyses indicate that linoleic acid may affect T47D cell growth by modulation of the estrogen receptor (ERalpha), the G13alpha G protein, and p38 MAP kinase gene expression as well genes involved in RNA transcription and cell cycle regulation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Division
  • Cell Line, Tumor
  • Cell Survival
  • Dose-Response Relationship, Drug
  • Estrogen Receptor alpha
  • GTP-Binding Protein alpha Subunits, G12-G13 / metabolism
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Linoleic Acid / pharmacology*
  • Mitogen-Activated Protein Kinases / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Oligonucleotides / chemistry
  • Polymerase Chain Reaction
  • RNA / metabolism
  • Receptors, Estrogen / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • S Phase
  • Transcription, Genetic
  • Up-Regulation
  • p38 Mitogen-Activated Protein Kinases

Substances

  • Estrogen Receptor alpha
  • Oligonucleotides
  • Receptors, Estrogen
  • RNA
  • Linoleic Acid
  • Mitogen-Activated Protein Kinases
  • p38 Mitogen-Activated Protein Kinases
  • GTP-Binding Protein alpha Subunits, G12-G13