Dipeptidase 1: A Candidate Tumor-Specific Molecular Marker in Colorectal Carcinoma

Cancer Lett. 2004 Jun 8;209(1):67-74. doi: 10.1016/j.canlet.2003.11.033.

Abstract

The aim of this study was to identify tumor-specific markers for the detection of rare disseminated colorectal tumor cells in peripheral venous blood and in intra-peritoneal saline lavage samples collected before and after resection of colorectal tumors. Using cDNA micro-array screening, we found dipeptidase 1 (DPEP1) to be highly expressed in colon tumors compared to matched normal mucosa. Relative reverse transcriptase (RT)-PCR showed that DPEP1 was over-expressed by >/=2 fold in colon tumor compared to normal colonic mucosal tissue in 56/68 (82%) patients. Using immunobead RT-PCR, a technique that first enriches for epithelial cells, we found DPEP1 positive cells in intra-peritoneal lavage and venous blood samples from 15/38 (39%) colorectal cancer cases. This is the first report of DPEP1 as a marker for disseminated colon tumor cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor*
  • Cell Line, Tumor
  • Colorectal Neoplasms / enzymology*
  • DNA, Complementary / metabolism
  • Dipeptidases / metabolism
  • Dipeptidases / physiology*
  • Epithelial Cells / metabolism
  • Female
  • GPI-Linked Proteins
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Metastasis
  • Oligonucleotide Array Sequence Analysis
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Biomarkers, Tumor
  • DNA, Complementary
  • GPI-Linked Proteins
  • Dipeptidases
  • dipeptidase
  • dipeptidase 1