The mammalian amiloride-insensitive non-specific salt taste receptor is a vanilloid receptor-1 variant

J Physiol. 2004 Jul 1;558(Pt 1):147-59. doi: 10.1113/jphysiol.2004.065656. Epub 2004 May 14.


The amiloride-insensitive salt taste receptor is the predominant transducer of salt taste in some mammalian species, including humans. The physiological, pharmacological and biochemical properties of the amiloride-insensitive salt taste receptor were investigated by RT-PCR, by the measurement of unilateral apical Na+ fluxes in polarized rat fungiform taste receptor cells and by chorda tympani taste nerve recordings. The chorda tympani responses to NaCl, KCl, NH4Cl and CaCl2 were recorded in Sprague-Dawley rats, and in wild-type and vanilloid receptor-1 (VR-1) knockout mice. The chorda tympani responses to mineral salts were monitored in the presence of vanilloids (resiniferatoxin and capsaicin), VR-1 antagonists (capsazepine and SB-366791), and at elevated temperatures. The results indicate that the amiloride-insensitive salt taste receptor is a constitutively active non-selective cation channel derived from the VR-1 gene. It accounts for all of the amiloride-insensitive chorda tympani taste nerve response to Na+ salts and part of the response to K+, NH4+ and Ca2+ salts. It is activated by vanilloids and temperature (> 38 degrees C), and is inhibited by VR-1 antagonists. In the presence of vanilloids, external pH and ATP lower the temperature threshold of the channel. This allows for increased salt taste sensitivity without an increase in temperature. VR-1 knockout mice demonstrate no functional amiloride-insensitive salt taste receptor and no salt taste sensitivity to vanilloids and temperature. We conclude that the mammalian non-specific salt taste receptor is a VR-1 variant.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amiloride / pharmacology*
  • Animals
  • Cations / metabolism
  • Chorda Tympani Nerve / physiology
  • Diuretics / pharmacology*
  • Female
  • Hot Temperature
  • Ion Channels / antagonists & inhibitors
  • Ion Channels / genetics
  • Ion Channels / physiology*
  • Mammals
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Minerals / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Salts / pharmacology*
  • TRPV Cation Channels
  • Taste / physiology*
  • Taste Buds / drug effects
  • Taste Buds / physiology*
  • Thermodynamics


  • Cations
  • Diuretics
  • Ion Channels
  • Minerals
  • Salts
  • TRPV Cation Channels
  • TRPV1 protein, mouse
  • Trpv1 protein, rat
  • Amiloride