Isolation and purification of honokiol and magnolol from cortex Magnoliae officinalis by high-speed counter-current chromatography

J Chromatogr A. 2004 May 21;1036(2):171-5. doi: 10.1016/j.chroma.2004.02.073.


High-speed counter-current chromatography was used to isolate and purify honokiol and magnolol from cortex Magnoliae Officinalis (Magnolia officinalis Rehd. et Wils.), a plant used in the traditional Chinese medicine. A crude sample, 150 mg, was successfully separated with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:0.4:1:0.4, v/v), and the fractions were analyzed by high-performance liquid chromatography. The separation produced 80 and 45 mg of honokiol and magnolol with purities of 99.2 and 98.2%, respectively, in 2.5 h.

MeSH terms

  • Biphenyl Compounds / isolation & purification*
  • Chromatography, High Pressure Liquid
  • Countercurrent Distribution / methods*
  • Lignans / isolation & purification*
  • Magnetic Resonance Spectroscopy
  • Magnolia / chemistry*


  • Biphenyl Compounds
  • Lignans
  • magnolol
  • honokiol