Regulation of phosphatidylcholine biosynthesis under salt stress involves choline kinases in Arabidopsis thaliana

FEBS Lett. 2004 May 21;566(1-3):115-20. doi: 10.1016/j.febslet.2004.04.015.


Increasing evidence suggests a major role for phosphatidylcholine (PC) in plant stress adaptation. The present work investigated the regulation of choline, PC and interconnected phosphatidylethanolamine biosynthesis in Arabidopsis thaliana L. as a function of cold- and salt- or mannitol-mediated hyperosmotic stresses. While PC synthesis is accelerated in both salt- and cold-treated plants, the choline kinase (CK) and phosphocholine cytidylyltransferase genes are oppositely regulated with respect to these abiotic treatments. Salt stress also stimulates CK activity in vitro. A possible regulatory role of CK in stimulating PC biosynthesis rate in salt-stressed plants is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / enzymology*
  • Arabidopsis / metabolism
  • Blotting, Northern
  • Carbon Radioisotopes
  • Choline Kinase / metabolism*
  • Cold Temperature
  • Cytidine Diphosphate Choline / metabolism
  • Cytosol / enzymology
  • Gene Expression
  • Hypertonic Solutions / pharmacology
  • Mannitol / pharmacology
  • Osmotic Pressure
  • Phosphatidylcholines / biosynthesis*
  • Phosphatidylcholines / genetics
  • Phosphatidylethanolamines / biosynthesis
  • Sodium Chloride / pharmacology*
  • Transcription, Genetic / drug effects


  • Carbon Radioisotopes
  • Hypertonic Solutions
  • Phosphatidylcholines
  • Phosphatidylethanolamines
  • phosphatidylethanolamine
  • Mannitol
  • Sodium Chloride
  • Cytidine Diphosphate Choline
  • Choline Kinase