The adenylate cyclase toxin from Bordetella pertussis--a novel promising vehicle for antigen delivery to dendritic cells

Int J Med Microbiol. 2004 Apr;293(7-8):571-6. doi: 10.1078/1438-4221-00291.


Bordetella pertussis secretes an adenylate cyclase toxin (CyaA or ACT) that targets primarily cells expressing the alphaMbeta2 integrin (CD11b/CD18) receptor. This toxin can deliver its N-terminal catalytic AC domain (400 amino acid residues) into the cytosol directly across the cytoplasmic membrane. Various heterologous CD8+, as well as CD4+ T-cell epitopes have been engineered into genetically detoxified CyaA and the resulting toxoids were successfully used as vectors for delivery of inserted epitopes into antigen-presenting cells. Upon processing and presentation, these recombinant CyaAs trigger specific MHC class I and/or class II-restricted T-cell responses both in vitro and in vivo.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Adenylate Cyclase Toxin / immunology*
  • Antigen Presentation / immunology
  • Antigens, Bacterial / immunology*
  • Bordetella Infections / immunology*
  • Bordetella Infections / prevention & control
  • Bordetella pertussis / enzymology
  • Bordetella pertussis / immunology*
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes
  • Dendritic Cells / immunology*
  • Epitopes, T-Lymphocyte / immunology
  • Histocompatibility Antigens Class I / immunology
  • Histocompatibility Antigens Class II / immunology
  • Humans


  • Adenylate Cyclase Toxin
  • Antigens, Bacterial
  • Epitopes, T-Lymphocyte
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II