Development of an Isotope-Coded Activity-Based Probe for the Quantitative Profiling of Cysteine Proteases

Bioorg Med Chem Lett. 2004 Jun 21;14(12):3131-4. doi: 10.1016/j.bmcl.2004.04.046.

Abstract

Quantification studies of complex protein mixtures have been restricted mainly to whole cell extracts. Here we describe the synthesis of two sets of isotope-coded activity-based probes that allow quantitative functional proteomics experiments on the cathepsins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cysteine Endopeptidases / analysis
  • Cysteine Endopeptidases / metabolism*
  • Isotope Labeling / methods*
  • Isotopes / chemical synthesis
  • Isotopes / metabolism
  • Proteomics / methods

Substances

  • Isotopes
  • Cysteine Endopeptidases