Purification and cell-surface marker characterization of quiescent satellite cells from murine skeletal muscle by a novel monoclonal antibody

Exp Cell Res. 2004 Jun 10;296(2):245-55. doi: 10.1016/j.yexcr.2004.02.018.


A novel monoclonal antibody, SM/C-2.6, specific for mouse muscle satellite cells was established. SM/C-2.6 detects mononucleated cells beneath the basal lamina of skeletal muscle, and the cells co-express M-cadherin. Single fiber analyses revealed that M-cadherin+ mononucleated cells attaching to muscle fibers are stained with SM/C-2.6. SM/C-2.6+ cells, which were freshly purified by FACS from mouse skeletal muscle, became MyoD+ in vitro in proliferating medium, and the cells differentiated into desmin+ and nuclear-MyoD+ myofibers in vitro when placed under differentiation conditions. When the sorted cells were injected into mdx mouse muscles, donor cells differentiated into muscle fibers. Flow cytometric analyses of SM/C-2.6+ cells showed that the quiescent satellite cells were c-kit-, Sca-1-, CD34+, and CD45-. More, SM/C-2.6+ cells were barely included in the side population but in the main population of cells in Hoechst dye efflux assay. These results suggest that SM/C-2.6 identifies and enriches quiescent satellite cells from adult mouse muscle, and that the antibody will be useful as a powerful tool for the characterization of cellular and molecular mechanisms of satellite cell activation and proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal* / biosynthesis
  • Antigens, Surface / analysis*
  • Biomarkers / analysis
  • Cadherins / analysis
  • Cell Culture Techniques
  • Cell Differentiation
  • Cell Separation / methods
  • Flow Cytometry
  • Immunohistochemistry
  • Mice
  • Mice, Inbred Strains
  • Muscle Fibers, Skeletal / cytology
  • Muscle, Skeletal / cytology*
  • Satellite Cells, Skeletal Muscle / cytology*
  • Satellite Cells, Skeletal Muscle / immunology*
  • Satellite Cells, Skeletal Muscle / transplantation


  • Antibodies, Monoclonal
  • Antigens, Surface
  • Biomarkers
  • Cadherins
  • M-cadherin