Presence of collagen IV in the ciliary zonules of the human eye: an immunohistochemical study by LM and TEM

J Histochem Cytochem. 2004 Jun;52(6):789-95. doi: 10.1369/jhc.4A6267.2004.

Abstract

The ciliary zonules of the eye are composed of fibrillar and non-fibrillar components. Fibrils provide tensile strength and elasticity, whereas non-fibrillar components serve as a coating surrounding the fibrils. This coating behaves as a barrier to macromolecules. The present light and transmission electron microscopic (LM and TEM) study identified collagen IV as a novel component of this coating. Collagen IV was demonstrated by pre-embedding and postembedding immunohistochemical (IHC) techniques using monoclonal and polyclonal antibodies. The specificity of the polyclonal anticollagen IV antibody was verified by ELISA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Child
  • Collagen Type IV / metabolism*
  • Enzyme-Linked Immunosorbent Assay
  • Eye / metabolism*
  • Eye / ultrastructure
  • Humans
  • Immunohistochemistry
  • Microscopy / methods
  • Middle Aged

Substances

  • Collagen Type IV